Diacylglycerol Guides the Hopping of Clathrin-Coated Pits along Microtubules for Exo-Endocytosis Coupling

分类： 生物学 >> 生物物理学 >> 细胞生物学 提交时间： 2016-05-11

Yuan, Tianyi Liu, Lin Wei, Lisi Zhao, Shiqun Zheng, Xiaolu Huang, Xiaoshuai Xie, Lihan Zong, Weijian Yang, Lu Liu, Yanmei Chen, Liangyi Yuan, Tianyi Liu, Lin Wei, Lisi Zhao, Shiqun Zheng, Xiaolu Huang, Xiaoshuai Xie, Lihan Zong, Weijian Yang, Lu

摘要： Many receptor-mediated endocytic processes are mediated by constitutive budding of clathrin-coated pits (CCPs) at spatially randomized sites before slowly pinching off from the plasma membrane (60100 s). In contrast, clathrin-mediated endocytosis (CME) coupled with regulated exocytosis in excitable cells occurs at peri-exocytic sites shortly after vesicle fusion (similar to 10 s). The molecular mechanism underlying this spatiotemporal coupling remains elusive. We show that coupled endocytosis makes use of preformed CCPs, which hop to nascent fusion sites nearby following vesicle exocytosis. A dynamic cortical microtubular network, anchored at the cell surface by the cytoplasmic linker-associated protein on microtubules and the LL5 beta/ELKS complex on the plasma membrane, provides the track for CCP hopping. Local diacylglycerol gradients generated upon exocytosis guide the direction of hopping. Overall, the CCP-cytoskeleton-lipid interaction demonstrated here mediates exocytosis-coupled fast recycling of both plasma membrane and vesicular proteins, and it is required for the sustained exocytosis during repetitive stimulations.

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